Vancomycin‐resistant enterococci in rooks (Corvus frugilegus) wintering throughout Europe
Identifieur interne : 000170 ( Main/Exploration ); précédent : 000169; suivant : 000171Vancomycin‐resistant enterococci in rooks (Corvus frugilegus) wintering throughout Europe
Auteurs : Veronika Oravcova [République tchèque] ; Anuradha Ghosh [États-Unis] ; Ludek Zurek [États-Unis] ; Jan Bardon [République tchèque] ; Sebastian Guenther [Allemagne] ; Alois Cizek [République tchèque] ; Ivan Literak [République tchèque]Source :
- Environmental Microbiology [ 1462-2912 ] ; 2013-02.
Abstract
This study's aims were to assess the prevalence of, and to characterize, vancomycin‐resistant enterococci (VRE) from rooks (Corvus frugilegus) wintering in Europe during 2010/2011. Faeces samples were cultivated selectively for VRE and characterized. Pulsed‐field gel electrophoresis and multilocus sequence typing (MLST) were used to examine epidemiologic relationships of vanA‐containing VRE. The vanA‐carrying VRE were tested in vitro for mobility of vancomycin resistance traits. VRE were found in 62 (6%) of 1073 rook samples. Enterococcal species diversity comprised Enterococcus gallinarum (48 isolates), followed by E. faecium (9) and E. faecalis (5). Eight VRE harboured the vanA and ermB genes. Seven vanA‐carrying VRE originated from the Czech Republic and one from Germany. All vanA‐carrying VRE were identified as E. faecium. Based on MLST analysis, six vanA‐positive isolates were grouped as ST92 type, one isolate belonged to ST121, and the remaining one was described as a novel type ST671. Seven out of eight isolates were able to transfer the vancomycin resistance trait via filter mating with a transfer rate of 8.95 ± 3.25 × 10−7 transconjugants per donor. In conclusion, wintering rooks in some European countries may disseminate clinically important enterococci and pose a risk for environmental contamination.
Url:
DOI: 10.1111/1462-2920.12002
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000F05
- to stream Istex, to step Curation: 000F04
- to stream Istex, to step Checkpoint: 000005
- to stream Main, to step Merge: 000171
- to stream Main, to step Curation: 000170
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Vancomycin‐resistant enterococci in rooks (Corvus frugilegus) wintering throughout Europe</title><author><name sortKey="Oravcova, Veronika" sort="Oravcova, Veronika" uniqKey="Oravcova V" first="Veronika" last="Oravcova">Veronika Oravcova</name></author><author><name sortKey="Ghosh, Anuradha" sort="Ghosh, Anuradha" uniqKey="Ghosh A" first="Anuradha" last="Ghosh">Anuradha Ghosh</name></author><author><name sortKey="Zurek, Ludek" sort="Zurek, Ludek" uniqKey="Zurek L" first="Ludek" last="Zurek">Ludek Zurek</name></author><author><name sortKey="Bardon, Jan" sort="Bardon, Jan" uniqKey="Bardon J" first="Jan" last="Bardon">Jan Bardon</name></author><author><name sortKey="Guenther, Sebastian" sort="Guenther, Sebastian" uniqKey="Guenther S" first="Sebastian" last="Guenther">Sebastian Guenther</name></author><author><name sortKey="Cizek, Alois" sort="Cizek, Alois" uniqKey="Cizek A" first="Alois" last="Cizek">Alois Cizek</name></author><author><name sortKey="Literak, Ivan" sort="Literak, Ivan" uniqKey="Literak I" first="Ivan" last="Literak">Ivan Literak</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:11E12ED44A05BC10779F2D70B5FCDF181CA31B85</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1111/1462-2920.12002</idno><idno type="url">https://api.istex.fr/document/11E12ED44A05BC10779F2D70B5FCDF181CA31B85/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000F05</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000F05</idno><idno type="wicri:Area/Istex/Curation">000F04</idno><idno type="wicri:Area/Istex/Checkpoint">000005</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000005</idno><idno type="wicri:doubleKey">1462-2912:2013:Oravcova V:vancomycin:resistant:enterococci</idno><idno type="wicri:Area/Main/Merge">000171</idno><idno type="wicri:Area/Main/Curation">000170</idno><idno type="wicri:Area/Main/Exploration">000170</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Vancomycin‐resistant enterococci in rooks (Corvus frugilegus) wintering throughout Europe</title><author><name sortKey="Oravcova, Veronika" sort="Oravcova, Veronika" uniqKey="Oravcova V" first="Veronika" last="Oravcova">Veronika Oravcova</name><affiliation wicri:level="3"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno</wicri:regionArea><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName></affiliation><affiliation></affiliation></author><author><name sortKey="Ghosh, Anuradha" sort="Ghosh, Anuradha" uniqKey="Ghosh A" first="Anuradha" last="Ghosh">Anuradha Ghosh</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS</wicri:regionArea><placeName><region type="state">Kansas</region></placeName></affiliation></author><author><name sortKey="Zurek, Ludek" sort="Zurek, Ludek" uniqKey="Zurek L" first="Ludek" last="Zurek">Ludek Zurek</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS</wicri:regionArea><placeName><region type="state">Kansas</region></placeName></affiliation></author><author><name sortKey="Bardon, Jan" sort="Bardon, Jan" uniqKey="Bardon J" first="Jan" last="Bardon">Jan Bardon</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>State Veterinary Institute, Olomouc</wicri:regionArea><wicri:noRegion>Olomouc</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc</wicri:regionArea><wicri:noRegion>Olomouc</wicri:noRegion></affiliation></author><author><name sortKey="Guenther, Sebastian" sort="Guenther, Sebastian" uniqKey="Guenther S" first="Sebastian" last="Guenther">Sebastian Guenther</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Microbiology and Epizootics, Veterinary Faculty, Free University Berlin, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Cizek, Alois" sort="Cizek, Alois" uniqKey="Cizek A" first="Alois" last="Cizek">Alois Cizek</name><affiliation wicri:level="3"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Infectious Diseases and Microbiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno</wicri:regionArea><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">République tchèque</country><wicri:regionArea>CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno</wicri:regionArea><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName></affiliation></author><author><name sortKey="Literak, Ivan" sort="Literak, Ivan" uniqKey="Literak I" first="Ivan" last="Literak">Ivan Literak</name><affiliation wicri:level="3"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno</wicri:regionArea><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">République tchèque</country><wicri:regionArea>CEITEC VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno</wicri:regionArea><placeName><settlement type="city">Brno</settlement><region>Moravie</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Environmental Microbiology</title><title level="j" type="abbrev">Environ Microbiol</title><idno type="ISSN">1462-2912</idno><idno type="eISSN">1462-2920</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-02">2013-02</date><biblScope unit="volume">15</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="548">548</biblScope><biblScope unit="page" to="556">556</biblScope></imprint><idno type="ISSN">1462-2912</idno></series><idno type="istex">11E12ED44A05BC10779F2D70B5FCDF181CA31B85</idno><idno type="DOI">10.1111/1462-2920.12002</idno><idno type="ArticleID">EMI12002</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1462-2912</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">This study's aims were to assess the prevalence of, and to characterize, vancomycin‐resistant enterococci (VRE) from rooks (Corvus frugilegus) wintering in Europe during 2010/2011. Faeces samples were cultivated selectively for VRE and characterized. Pulsed‐field gel electrophoresis and multilocus sequence typing (MLST) were used to examine epidemiologic relationships of vanA‐containing VRE. The vanA‐carrying VRE were tested in vitro for mobility of vancomycin resistance traits. VRE were found in 62 (6%) of 1073 rook samples. Enterococcal species diversity comprised Enterococcus gallinarum (48 isolates), followed by E. faecium (9) and E. faecalis (5). Eight VRE harboured the vanA and ermB genes. Seven vanA‐carrying VRE originated from the Czech Republic and one from Germany. All vanA‐carrying VRE were identified as E. faecium. Based on MLST analysis, six vanA‐positive isolates were grouped as ST92 type, one isolate belonged to ST121, and the remaining one was described as a novel type ST671. Seven out of eight isolates were able to transfer the vancomycin resistance trait via filter mating with a transfer rate of 8.95 ± 3.25 × 10−7 transconjugants per donor. In conclusion, wintering rooks in some European countries may disseminate clinically important enterococci and pose a risk for environmental contamination.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>République tchèque</li><li>États-Unis</li></country><region><li>Berlin</li><li>Kansas</li><li>Moravie</li></region><settlement><li>Berlin</li><li>Brno</li></settlement></list><tree><country name="République tchèque"><region name="Moravie"><name sortKey="Oravcova, Veronika" sort="Oravcova, Veronika" uniqKey="Oravcova V" first="Veronika" last="Oravcova">Veronika Oravcova</name></region><name sortKey="Bardon, Jan" sort="Bardon, Jan" uniqKey="Bardon J" first="Jan" last="Bardon">Jan Bardon</name><name sortKey="Bardon, Jan" sort="Bardon, Jan" uniqKey="Bardon J" first="Jan" last="Bardon">Jan Bardon</name><name sortKey="Cizek, Alois" sort="Cizek, Alois" uniqKey="Cizek A" first="Alois" last="Cizek">Alois Cizek</name><name sortKey="Cizek, Alois" sort="Cizek, Alois" uniqKey="Cizek A" first="Alois" last="Cizek">Alois Cizek</name><name sortKey="Literak, Ivan" sort="Literak, Ivan" uniqKey="Literak I" first="Ivan" last="Literak">Ivan Literak</name><name sortKey="Literak, Ivan" sort="Literak, Ivan" uniqKey="Literak I" first="Ivan" last="Literak">Ivan Literak</name></country><country name="États-Unis"><region name="Kansas"><name sortKey="Ghosh, Anuradha" sort="Ghosh, Anuradha" uniqKey="Ghosh A" first="Anuradha" last="Ghosh">Anuradha Ghosh</name></region><name sortKey="Zurek, Ludek" sort="Zurek, Ludek" uniqKey="Zurek L" first="Ludek" last="Zurek">Ludek Zurek</name></country><country name="Allemagne"><region name="Berlin"><name sortKey="Guenther, Sebastian" sort="Guenther, Sebastian" uniqKey="Guenther S" first="Sebastian" last="Guenther">Sebastian Guenther</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/RenardV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000170 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000170 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= RenardV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:11E12ED44A05BC10779F2D70B5FCDF181CA31B85
|texte= Vancomycin‐resistant enterococci in rooks (Corvus frugilegus) wintering throughout Europe
}}
| This area was generated with Dilib version V0.6.27. |  |